Day 1 :
Time : 09:00-09:40
Nongyue He has completed his PhD at the age of 39 years from Nanjing University and postdoctoral studies from Southeast University School of Biological Science and Medical Engineering. He is the director of Hunan Key Laboratory of Biomedical Nanomaterials and Devices at Hunan University of Technology. He has published more than 300 papers in reputed journals and has been serving as an co-editor-in-chief of Materials Express.
Aptamers are single-stranded DNAs or RNAs specifically binds to various targets, which show great potential in the early diagnostics and therapy of cancer, due to their superiority over antibodies. Molecular subtyping of breast cancer remains a challenge in clinic medicine, which plays a crucial role in the treatment. In this study, four ssDNA aptamers against MCF-7 breast cancer cells were developed by Cell-SELEX for differentiating breast cancer subtypes. Among all these aptamers, aptamer MF3 showed better specificity than other apatmers as well as favorable binding affinity towards MCF-7 cells with a Kd value of 82.25 ± 25.14 nM. Aptamer MF3 can not only successfully distinguish MCF-7 breast cancer cells from MDA-MB-231, SK-BR-3 breast cancer cells and MCF-10A human normal mammary epithelial cells, but also can differentiate MCF-7 cells from other cancer cells or normal cells, such as A549 lung cancer cells, HepG2 hepatocellular carcinoma cells, L02 normal hepatic cells, HeLa cells, A375 melanoma cells, SGC-7901 gastric carcinoma cells and GES-1 gastric epithelial cells. Moreover, the results of in vivo fluorescence imaging of tumor-bearing mice indicated that aptamer MF3 can distinguish the xenografts of MCF-7 cells tumor-bearing mice from that of MDA-MB-231 and SK-BR-3 cells tumor-bearing mice in vivo. Finally, the bio-distribution of aptamer MF3 also suggested its potential application for the targeted therapy of breast cancer. Collectively, all these results demonstrated aptamer MF3 a promising highly-effective theranostic tool for the personalized medicine of breast cancer
Faculty of Science
Keynote: Antifungal Activity of Loaded Chitosan Nanoparticles with S-nitrosomercaptosuccinic acid against Candida sp.
Time : 09:40-10:10
Gabriela Morón is a Pharmaceutical Chemistry graduated from the Faculty of Science of the Cayetano Heredia Peruvian University. She has won the Prize for Innovation in Pharmaceutical Technology awarded by the Association of National Pharmaceutical Industries from 2015 to 2016. Currently, she works in the Cosmetic Industry as Technical Director and is pursuing a specialization in Lean Six Sigma Black Belt by the University of the Pacific.
Introduction: Candida sp species are fungal pathogens that affect patients with risk pathologies. Due to the change in their conventional drug susceptibility patterns, it is necessary to investigate therapeutic alternatives. It is proposed to evaluate the antifungal potential of nitric oxide (NO), by administering it in the donor s-nitrosomercaptosuccinic acid (MSA-NO), encapsulated in chitosan nanoparticles (Np) to improve its bioavailability and inhibit the growth of Candida albicans, glabrata, krusei and parapsilosis.
Methods: Three batches of nanoparticles loaded with mercaptosuccinic acid (MSA-Np) were synthesized by ionic gelation. The effective particle diameter and polydispersity index were analyzed by dynamic light scattering and encapsulation efficiency by the Ellman reaction. After adding NaNO2, MSA-NO Np. The minimum inhibitory concentration (MIC) against species of Candida sp. iwas determined by microdilution and the NO release profile was estimated by UV spectrophotometry.
Results: The MSA Np presented optimal values of effective particle diameter (241.69 ± 18.95 nm), polydispersity index (0.274 ± 0.015) and encapsulation efficiency (97.52 ± 0.07%). The MIC values of C. glabrata and C. albicans were 0.28 mg / mL and 2.25 mg / mL, respectively. The lowest CMI corresponded to C. krusei while C. albicans was the least susceptible to NO. The results did not vary significantly batch to batch.
Conclusions: A procedure of synthesis of MSA-NO Np with antifungal activity on Candida sp was validated. The antifungal potency varied according to the species. The chitosan of MSA-NO Np was useful as a polymer matrix for NO controlled release